Technical Data
I1900-02E3
IgE (HRP)
Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry (frozen), and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:200-1:500
ELISA: 1:10,000-1:100,000
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceCanineGoat
Concentration:
~1mg/ml
Immunogen:
Canine IgE.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% proclin TM300, 0.01% thimerosal, 0.2% BSA. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes canine IgE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.