Technical Data
IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150ng/ml, compared to 10mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Suitable for use in Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 4ug/ml
ELISA: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG18L344Highly Purified
100ug-20CBlue IceHumanMouse
Purified human IgE
Purified by Ion Exchange chromatography
Supplied as a liquid in TBS, pH 8.0, 15mM sodium azide
Recognizes human IgE. Specificity was confirmed by ELISA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.