Technical Data
I1900-11B
IgE
Description:
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion. But it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies; and the life-threatening respiratory distress of asthma and anaphylactic shock.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:500 coating
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanChicken
Concentration:
~1mg/ml
Immunogen:
Human IgE purified from myeloma plasma.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Reacts with IgE by ELISA, IEP and gel diffusion analysis. Serum known to be very low in IgE shows no reaction with the anti IgE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.