Technical Data
Suitable for use in Immunohistochemistry, ELISA and
Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry : 1:500-1:2500
ELISA: 1:5000-1:50,000. Assayed against 1ug of Rat IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT.
Western Blot: 1:1000-1:10,000
Optimal working dilutions to be determined by researcher.

Label Molecular Weight: 40,000

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
PabIgGAffinity Purified
2mg-20CBlue IceRatSheep
~2mg/ml (after reconstitution)
Rat IgG whole molecule
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Recognizes rat IgE. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum, rat IgG and rat serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Conjugation References:vModified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.