Technical Data
I1903-10E
IgG, IgA, IgM, H&L (TRITC)
Description:
Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)

Absorption Wavelength: 550 nm

Emission Wavelength: 570 nm

Fluorochrome/Protein Ratio: 2.6 moles TRITC per mole of Goat IgG


Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution: Optimal dilutions to be determined by the researcher.

Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceRatGoat
Concentration:
~1mg/ml (UV280)
Immunogen:
Rat IgG whole molecule
Purity:
Purified by immunoaf nity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Labeled with TRITC.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA, Rat IgG and Rat IgM. This reagent is suitable for the detection of all rat immunoglobulin subclases, isotypes and chain combinations.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.