Technical Data
I1903-14R
IgG, H&L (HRP)
Description:
Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1: 5000 -1:50,000, assayed against 1.0ug of Rat IgG in a standard capture using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
Immunohistochemistry: 1:500-1:2500
Western Blot: 1:1000-1:10,000
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceRatSheep
Concentration:
~2mg/ml
Immunogen:
Rat IgG whole molecule
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with HRP.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rat IgG and Rat Serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.