Description:
Applications:
Suitable for use in Immunoblot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:3000-1:44,000. 1.0ug of Canine IgG was used in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | 4°C Do Not Freeze | Blue Ice | Canine | Rabbit |
|
| Concentration: |
| ~0.7mg/ml |
| Immunogen: |
| Canine IgG F(c) fragment |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD ) |
| Specificity: |
| Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Canine IgG, Canine IgG F(c) and Canine Serum. No reaction was observed against Canine IgG F(ab’)2. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971
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