Technical Data
I1903-17D
IgG, Fc (AP)
Description:
Applications:
Suitable for use in Immunoblot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:3000-1:44,000. 1.0ug of Canine IgG was used in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceCanineRabbit
Concentration:
~0.7mg/ml
Immunogen:
Canine IgG F(c) fragment
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD )
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Canine IgG, Canine IgG F(c) and Canine Serum. No reaction was observed against Canine IgG F(ab)2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971