Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. Other applications not tested.
ELISA (standard capture): 1:500 to 1:2,000, 1.0 ug of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|1mg||4°C Do Not Freeze||Blue Ice||Equine||Rabbit|
| ~1.0 mg/ml|
|horseIgG F(c) fragmentLabel: Alkaline Phosphatase (Calf Intestine) (MW)140kD)|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0 0.01% Sodium Azide, 10 mg/ml BSA (IgG and Protease free)|
| Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab’)2.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.|