Technical Data
IgG, Fc (AP)
Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA (standard capture): 1:500 to 1:2,000, 1.0 ug of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
1mg4C Do Not FreezeBlue IceEquineRabbit
~1.0 mg/ml
horseIgG F(c) fragment Label: Alkaline Phosphatase (Calf Intestine) (MW)140kD)
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0 0.01% Sodium Azide, 10 mg/ml BSA (IgG and Protease free)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab)2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.