Description:
Applications:
Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA (standard capture): 1:500 to 1:2,000, 1.0 ug of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | 4°C Do Not Freeze | Blue Ice | Equine | Rabbit |
|
| Concentration: |
| ~1.0 mg/ml |
| Immunogen: |
| horseIgG F(c) fragment Label: Alkaline Phosphatase (Calf Intestine) (MW)140kD) |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0 0.01% Sodium Azide, 10 mg/ml BSA (IgG and Protease free) |
| Specificity: |
| Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab’)2. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
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