Technical Data
I1903-28
IgG (AP)
Description:
Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:8300
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Substrate: N2600-10 RT for 30 minutes.

Label:
Alkaline phoshpatase (calf intestine) (MW 14kD)

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug4C Do Not FreezeBlue IceGoatDonkey
Concentration:
~1mg/ml
Immunogen:
Goat IgG whole molecule.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:
Recognizes goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-donkey serum, goat IgG and goat serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.