Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Western Blot: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Substrate: N2600-10 RT for 30 minutes.
Alkaline phoshpatase (calf intestine) (MW 14kD)
Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
|500ug||4°C Do Not Freeze||Blue Ice||Goat||Donkey|
| ~1mg/ml |
|Goat IgG whole molecule.|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.09% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).|
|Recognizes goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-donkey serum, goat IgG and goat serum.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|