Technical Data
I1903-34E
IgG, H&L (HRP)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:25,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with 1 ml sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceHamsterGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Armenian hamster IgG whole molecule.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes Armenian hamster IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, Armenian hamster IgG and Armenian hamster serum. Greatly diminished reactivity will occur against Golden Syrian hamster IgG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
11. Farr & Nakane, J. Immunol. Methods 47: 129-144 (1981).