Suitable for use in ELISA and Immunofluorescence. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 4.3 moles FITC per mole of Rabbit IgG
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Human IgG y gamma chain|
|Purified by immunoaffinity chromatography.|
|Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW 390 daltons)|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Human IgA or Human IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Human heavy or light chain isotypes. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|