Technical Data
I1903-35D
IgG (FITC)
Description:
Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 4.3 moles FITC per mole of Rabbit IgG

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Human IgG y gamma chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW 390 daltons)
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Human IgA or Human IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.