Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Human kappa light chain|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in 0.1M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide. No stabilizing proteins added.|
|Recognizes human IgG, kappa. Assay by IEP resulted in a single precipitin arc against anti-goat serum, human IgG and human serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at <1% crossreactivity against other human heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|