Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions:
ELISA: 1:25,000
Immunohistochemistry: 1:1000-1:5000
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | F(ab’)2 | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | -20°C | Blue Ice | Human | Goat |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Human kappa light chain |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in 0.1M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide. No stabilizing proteins added. |
| Specificity: |
| Recognizes human IgG, kappa. Assay by IEP resulted in a single precipitin arc against anti-goat serum, human IgG and human serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at <1% crossreactivity against other human heavy or light chain isotypes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
|
|
