Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.
Optimal dilutions to be determined by the researcher.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Human (kappa) light chain|
|Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |
|Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|