Technical Data
I1903-37Q
IgG (kappa chain specific) (AP)
Description:
Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Label:
Alkaline Phosphatase (Calf Intestine), M.W.-140,000D

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabF(ab’)2Affinity Purified
SizeStorageShippingSourceHost
500ug4°C Do Not FreezeBlue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Human kappa chain
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Alkaline Phosphatase.
Specificity:
Recognizes human IgG (kappa chain specific). No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.