Technical Data
I1903-37S
IgG (lambda chain specific) (Biotin)
Description:
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:4000-1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Human IgG (lambda) light chain.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes the lambda chain of human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.