Description:
Applications:
Suitable for use in ELISA and Immunofluorescence and Fluorescent Western Blot. Other applications have not been tested.
Recommended Dilution:
FLISA: 1:10,000 -1:50,000
Immunofluoresence: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Excitation Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 1.8 moles FITC per mole of Goat IgG Fab
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | F(ab’)2 | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | -20°C | Blue Ice | Human | Goat |
|
| Concentration: |
| ~1mg/ml (after reconstitution) |
| Immunogen: |
| Human (lambda light chain) |
| Purity: |
| Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |
| Form |
| Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 0.01% sodium azide, and 10mg/ml IgG and protease free bovine serum albumin (BSA). Labeled with Fluorescein isothiocyanate (FITC; MW= 390 kD). Reconstitute with 1ml deionized water. |
| Specificity: |
| Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
|
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