Technical Data
I1903-38V
IgG (lambda chain specific) (FITC)
Description:
Applications:
Suitable for use in ELISA (Fluorescent) (Fluorescent) and Immunofluorescence and Fluorescent Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA (Fluorescent): 1:10,000 -1:50,000
Immunofluoresence: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Excitation Wavelength: 495nm
Emission Wavelength: 528nm

Fluorochrome/Protein Ratio: 1.8 moles FITC per mole of Goat IgG Fab

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human (lambda light chain)
Purity:
Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form
Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 0.01% sodium azide, and 10mg/ml IgG and protease free bovine serum albumin (BSA). Labeled with Fluorescein isothiocyanate (FITC; MW= 390 kD). Reconstitute with 1ml deionized water.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.