Technical Data
IgG (lambda chain specific) (FITC)
Suitable for use in FLISA (Fluorescent) (Fluorescent) and Immunofluorescence and Fluorescent Western Blot. Other applications have not been tested.

Recommended Dilution:
FLISA (Fluorescent): 1:10,000 -1:50,000
Immunofluoresence: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Excitation Wavelength: 495nm
Emission Wavelength: 528nm

Fluorochrome/Protein Ratio: 1.8 moles FITC per mole of Goat IgG Fab

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabF(ab)2Affinity Purified
1mg-20CBlue IceHumanGoat
~1mg/ml (after reconstitution)
Human (lambda light chain)
Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 0.01% sodium azide, and 10mg/ml IgG and protease free bovine serum albumin (BSA). Labeled with Fluorescein isothiocyanate (FITC; MW= 390 kD). Reconstitute with 1ml deionized water.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.