Suitable for use in ELISA (Fluorescent) (Fluorescent) and Immunofluorescence and Fluorescent Western Blot. Other applications have not been tested.
ELISA (Fluorescent): 1:10,000 -1:50,000
Optimal dilutions to be determined by the researcher.
Excitation Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 1.8 moles FITC per mole of Goat IgG Fab
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| ~1mg/ml (after reconstitution)|
|Human (lambda light chain)|
|Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |
|Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 0.01% sodium azide, and 10mg/ml IgG and protease free bovine serum albumin (BSA). Labeled with Fluorescein isothiocyanate (FITC; MW= 390 kD). Reconstitute with 1ml deionized water.|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|