Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:200 to 1:1000. 1.0ug of Human IgG was used in a standard capture ELISA with pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | F(ab’)2 | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 500ug | | 4°C Do Not Freeze | Blue Ice | Human | Goat |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Human lambda chain. MW of label: 140kD |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M Zznc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with alkaline phosphatase (AP). |
| Specificity: |
| Recognizes human lambda light chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
|
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