Technical Data
I1903-50K
IgE (HRP)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:200-1:5000
Immunohistochemistry: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Purified human IgE heavy chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP). Reconstitute with 1.1ml sterile ddH2O.
Specificity:
Recognizes heavy chain of human IgE. No reactivity has been observed with non-IgE human serum immunogloublins and with light chains on all human immunoglobulins. Does not react with serum proteins from bovine, mouse or rabbit
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.