		Technical Data
I1903-58E	IgG, IgA, IgM, H&L, X-adsorbed (TRITC)

Description:
Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label: Tetramethylrhodamine isothiocyanante (TRITC)
Molecular Weight: 444 daltons
Absorption Wavelength: 550nm
Emission Wavelength: 570nm
Fluorochrome/Protein Ratio: 3.9 moles TRITC per mole of Goat IgG

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	F(ab’)2		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Human	Goat

Concentration:

~1mg/ml

Immunogen:

Human IgG, IgA, and IgM whole molecules

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free),0.01% sodium azide. Labeled with TRITC.

Specificity:

Recognizes Human IgG, IgA and IgM (all Human immunoglobulin subclasses and chain combinations). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rhodamine and anti-goat serum. No reaction was detected against anti-goat IgG F(c), anti-pepsin or other human serum proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.