Technical Data
I1903-58G5
IgG IgA IgM, H&L, X-adsorbed (Texas Red)
Description:
Applications:
Suitable for use in Immunofluorescence Microscopy, ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunofluorescence Microscopy: 1:1000-1:5000
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Excitation Wavelength:
596nm

Emission Wavelength:
620nm

Fluorochrome/Protein Ratio:
3moles Texas Red per Goat IgG F(ab)2

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Texas Red conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Human IgG, IgA and IgM whole molecules
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red (MW: 625). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes Human IgG, IgA and IgM. Detects all human immunoglobulin classes, isotype and chain combinations. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum. No reaction was observed against anti-goat IgG F(c), anti-pepsin or mouse serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.