		Technical Data
I1903-58G5	IgG IgA IgM, H&L, X-adsorbed (Texas Red)

Description:
Applications:
Suitable for use in Immunofluorescence Microscopy, ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunofluorescence Microscopy: 1:1000-1:5000
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Excitation Wavelength:
596nm

Emission Wavelength:
620nm

Fluorochrome/Protein Ratio:
3moles Texas Red per Goat IgG F(ab’)2

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Texas Red® conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	F(ab’)2		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Human	Goat

Concentration:

~1mg/ml

Immunogen:

Human IgG, IgA and IgM whole molecules

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red (MW: 625). Reconstitute with 1ml sterile ddH2O.

Specificity:

Recognizes Human IgG, IgA and IgM. Detects all human immunoglobulin classes, isotype and chain combinations. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum. No reaction was observed against anti-goat IgG F(c), anti-pepsin or mouse serum proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.