Technical Data
I1903-58G6
IgG, IgA, IgM, H&L, X-adsorbed (FITC)
Description:
Applications:
Suitable for use in ELISA (Fluorescent), Fluorescent Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 495nm

Emission Wavelength: 528nm

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceMouseGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Mouse IgG, IgA, and IgM whole molecules
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes mouse IgG, IgA, and IgM (all isotypes and chain combinations). Assay by IEP resulted in a single precipitin arc against anti-fluorescein and anti-goat serum. Does not crossreact with anti-goat IgG F(c), anti-pepsin or human serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.