Technical Data
IgG, Fc
Suitable for use in ELISA.

Recommended Dilution:
Direct ELISA: 1:10,000 dilution will yield 0.75 O.D. using alkaline phosphatase-conjugated rabbit anti-mouse Ig

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
500ug4C (-20C Glycerol)Blue IceHumanMouse
Purified human IgG
Purified by Ion Exchange chromatography.
Supplied as a liquid in PBS, 40% glycerol
Recognizes human IgG-Fc. Does not react with Fab or F(ab')2 of IgG. Some crossreaction with IgG of other species such as equine and sheep was observed. No crossreaction with human IgA, IgE or IgM.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The presence of keratin 5 as an IgG Fc binding protein in human corneal epithelium. Endo K, Kawasaki S, Kinoshita S, Exp Eye Res 2004 Jun;78(6):1137-41 2. Synthesis and receptor binding of IgG1 peptides derived from the IgG Fc region. Uray K, Medgyesi D, Sarmay G, J Mol Recognit 2004 Mar-Apr;17(2):95-105 3. Association between polymorphism in IgG Fc receptor IIIa coding gene and biological response to infliximab in Crohn's disease. Louis E, El Ghoul Z, Dall'Ozzo S, De Vos M, Aliment Pharmacol Ther 2004 Mar 1;19(5):511-9 4. New insights into the pathophysiology and in vivo function of IgG Fc receptors through gene deletion studies. Baumann U, Schmidt RE, Arch Immunol Ther Exp (Warsz) 2003;51(6):399-406 5. [Fc receptors of endothelial cells of cardiac valves: comparison of IgG Fc binding activity of these receptors and of Fc receptors of group A streptococci] Danilova TA, Zh Mikrobiol Epidemiol Immunobiol 2003 Mar-Apr;(2):46-51 6. IgG Fc receptor polymorphisms in human disease: implications for intravenous immunoglobulin therapy. Binstadt BA, Geha RS, J Allergy Clin Immunol 2003 Apr;111(4):697-703.