Technical Data
I1903-80A
IgY, H&L, X-Adsorbed, 18nm (Gold)
Description:
Applications:
Suitable for use in ELISA, Immunoelectrophoresis, Western Blot, Electron Microscopy and Light Microscopy. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10-1:20
Immunoelectrophoresis: 1:10-1:20
Western Blot: 1:10-1:20
Electron Microscopy: 1:10-1:20
Light Microscopy: 1:10-1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.

Also Availbable:
I1903-80: IgY, H&L, X-Adsorbed, 12nm (Gold) Pab Dk xCh
I1903-80A: IgY, H&L, X-Adsorbed, 18nm (Gold) Pab Dk xCh
I1903-80B: IgY, H&L, X-Adsorbed, 6nm (Gold) Pab Dk xCh
I1906: IgY Pab Rb xCh
I1906-01: IgY, Fc (HRP) Pab Gt xCh
I1906-10: IgY (HRP) Pab Rb xCh
I1906-12: IgY (FITC) Pab Rb xCh
I1906-14: IgY (AP) Pab Rb xCh
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
300ul4C Do Not FreezeBlue IceChickenDonkey
Concentration:
Not determined
Immunogen:
Chicken IgY
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.01M sodium borate-sodium phosphate, 0.15M sodium chloride, pH 8.5, 15mg/ml BSA (IgG and Protease Free), 0.05% sodium azide. Labeled with 18nm Colloidal Gold. OD(520) = 2.0.
Specificity:
Recognizes whole molecule chicken IgY and the light chains of chicken immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Exhibits minimal crossreactivity with bovine, goat, guinea pig, syrian hamster, equine, human, mouse, rabbit, rat and sheep serum proteins. May crossreact with immunoflobulins from other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Faulk, W.P., Taylor, G.M.: An Immunocolloid, Method for the Electron Microscope. Immunochemistry 8: 081-1983 (1971). 2. Chandler, J., Gurmin, T., Robinson, N.: The Place of Gold in Rapid Tests. IVD Technology 6(2): 37-49 (2000). 3. Hayat, M.A. ed.: Colloidal Gold: Principles, Methods, and Applications, vol. 1 (1989). Academic Press, San Diego. 4. Handley, D.A.: Methods for Synthesis of Colloidal Gold. page 22, in Hayat, M.A., ed. Colloidal Gold: Principles, Methods, and Applications (1989). Academic Press, San Diego. 5. Frens, G.: Controlled Nucleation for the Regulation of Particle Size in Monodisperse Gold Solutions. Nature Physical Science 20: 241 (1973).