Suitable for use in ELISA, Immunoelectrophoresis, Western Blot, Electron Microscopy and Light Microscopy. Other applications not tested.
Western Blot: 1:10-1:20
Electron Microscopy: 1:10-1:20
Light Microscopy: 1:10-1:20
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.
|300ul||4°C Do Not Freeze||Blue Ice||Chicken||Donkey|
| Not Determined|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in 0.01M sodium borate-sodium phosphate, 0.15M sodium chloride, pH 8.5, 15mg/ml BSA (IgG and Protease Free), 0.05% sodium azide. Labeled with 18nm Colloidal Gold. OD(520) = 2.0.|
|Recognizes whole molecule chicken IgY and the light chains of chicken immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Exhibits minimal crossreactivity with bovine, goat, guinea pig, Syrian hamster, equine, human, mouse, rabbit, rat and sheep serum proteins. May crossreact with immunoflobulins from other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Faulk, W.P. & Taylor, G.M., Immunochemistry 8: 1081-1083 (1971). 2. Chandler, J., et al., IVD Technology 6(2): 37-49 (2000). 3. Hayat, M.A., ed. 1989. Colloidal Gold: Principles, Methods, and Applications, vol. 1. San Diego: Academic Press. 4. Handley, D.A., 1989. Methods for Synthesis of Colloidal Gold. page 22 in Hayat, M.A., ed. Colloidal Gold: Principles, Methods, and Applications. San Diego: Academic Press. 5. Frens, G., Nature Physical Science. 20: 241 (1973).|