Technical Data
IgG, IgA, IgM, H&L, F(ab)2 Fragment (AP)
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:500-1:2000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
PabIgGAffinity Purified
500ug4CBlue IceHumanGoat
As reported
Human IgG, IgA, IgM
~90% based on SDS-PAGE, may contain small amounts of intact IgG.
Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zinc chloride, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
Recognizes heavy and light on human IgG, IgA, IgM based on IEP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.