Technical Data
I1904-28W
IgG, Fc (AP)
Description:
Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunhistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:400 to 1:2,800 using 1.0 ug of Goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.

Label:
Alkaline Phosphatase (Calf Intestine) (MW 140kD)

Storage and Stability:
Store at 2-8°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. AP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4°C Do Not FreezeBlue IceGoatRabbit
Concentration:
~1mg/ml
Immunogen:
Goat IgG F(c) fragment
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab’).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.