Technical Data
I1904-34T
IgG (HRP)
Description:
Applications:
Suitable for Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000-1:10,000
ELISA: 1:10,000-1:100,000
Immunohistochemistry: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human IgG gamma heavy chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (Protease and IgG free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum. Specificity was confirmed by ELISA, 1% crossreactivity against other human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Farr & Nakane, J. Immunol. Methods 47: 129-144, (1981).