Technical Data
I1904-34T
IgG (HRP)
Description:
Applications:
Suitable for Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000-1:10,000
ELISA: 1:10,000-1:100,000
Immunohistochemistry: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human IgG gamma heavy chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (Protease and IgG free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum. Specificity was confirmed by ELISA, 1% crossreactivity against other human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Farr & Nakane, J. Immunol. Methods 47: 129-144, (1981).