Technical Data
I1904-34Z
IgG, Fc (AP)
Description:
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000-1:20,000, using pNPP, p-nitrophenyl phosphate, as a substrate for 30 minutes at room temperature.
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000.
Optimal dilutions to be determined by the researcher.

Label:
Alkaline Phosphatase (Calf Intestine).

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4°C Do Not FreezeBlue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Human IgG F(c) fragment
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).
Specificity:
Recognizes Fc portion of human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against Human IgG F(ab’)2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.