Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.
Optimal dilutions to be determined by the researcher.
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength: 550nm
Emission Wavelength: 570nm
Fluorochrome/Protein Ratio: 4.0 moles TRITC per mole of Goat IgG F(ab’)2
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| 2.5 mg/ml (by UV absorbance at 280 nm)|
|Human (lambda chain)|
|Purified by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation|
|Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free),0.01% Sodium Azide. Labeled with TRITC.|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti- Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|