Technical Data
I1904-35M
IgG, IgA, IgM, H&L, X-adsorbed (TRITC)
Description:
Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label:
Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons)

Absorption Wavelength: 550nm

Emission Wavelength: 570nm

Fluorochrome/Protein Ratio: 3.6 moles TRITC per mole of Goat IgG F(ab)2

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceMouseGoat
Concentration:
~1mg/ml
Immunogen:
Mouse IgG, IgA, and IgM whole molecules
Purity:
Purified by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide. Labeled with TRITC.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.