Technical Data
I1904-35N
IgG, IgA, IgM, H&L, X-adsorbed (Biotin)
Description:
Applications:
Suitable for Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:500-1:2000
Optimal dilution to be determined by the researcher.

Label: Biotinamidocaproate N-Hydroxysuccinimide Ester(BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Companion Products:
A0030: ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO
S7973-97A: Streptavidin (HRP)

Also Available:
I1904-04B: IgG, H&L (Biotin) Pab Gt xMo
I1904-06B: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo
I1904-06G: IgG, H&L, X-Adsorbed (Biotin) Pab Gt xMo
I1904-09S: IgG, H&L (Biotin) Pab Gt xMo
I1904-09T: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-09U: IgG, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-14B: IgG, Fc (Biotin) Pab Gt xMo
I1904-14J: IgG, Fc, X-adsorbed (Biotin) Pab Gt xMo
I1904-19B: IgG, F(ab)2 (Biotin) Pab Gt xMo
I1904-25B: IgG, IgA, IgM H&L (Biotin) Pab Gt xMo
I1904-35N: IgG, IgA, IgM, H&L, X-adsorbed (Biotin) Pab Gt xMo
I1904-67QIgG F(ab)2, X-adsorbed (Biotin) Pab Gt xMo


TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceMouseGoat
Concentration:
~1mg/ml
Immunogen:
Mouse IgG, IgA and IgM whole molecules
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long-term storage.. Labeled with Biotin.
Specificity:
Recognizes Mouse IgG, IgA and IgM, whole molecules (all subclasses, isotypes and chain combinations). Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Species Crossreactivity: Does not crossreact with human serum protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.