Technical Data
I1904-35P
IgG, IgA, IgM, H&L, X-adsorbed (AP)
Description:
Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:6300
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:100-1:500
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabF(ab’)2Affinity Purified
SizeStorageShippingSourceHost
500ug4°C Do Not FreezeBlue IceMouseGoat
Concentration:
~1mg/ml
Immunogen:
Mouse IgG, IgA and IgM whole molecules.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase.
Specificity:
Recognizes mouse IgG, IgA, IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-goat serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.