Technical Data
I1904-35P
IgG, IgA, IgM, H&L, X-adsorbed (AP)
Description:
Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:6300
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:100-1:500
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C as an undiluted liquid for 12 months. Dilute only prior to immediate use. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabF(ab)2Affinity Purified
SizeStorageShippingSourceHost
500ug4C Do Not FreezeBlue IceMouseGoat
Concentration:
~1mg/ml
Immunogen:
Mouse IgG, IgA and IgM whole molecules.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase.
Specificity:
Recognizes mouse IgG, IgA, IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-goat serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.