Description:
Applications:
Suitable for use in Dot Blot, Western Blot, ELISA, Immunoelectrophoresis and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:500-1:3000
ELISA: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. AP conjugates are sensitive to light.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | 4°C Do Not Freeze | Blue Ice | Human | Goat |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Human IgG, IgA and IgM whole molecule. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (Calf Intestine), MW: 140kD. |
| Specificity: |
| Recognizes human IgG, IgA and IgM (all human immunoglobulin subclasses and chain combinations). Assay by IEP resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-goat serum. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
|
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