Technical Data
I1904-35X
IgG, Kappa (Biotin)
Description:
Applications:
Suitable for use in Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:15000-1:75,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4°C (-20°C Glycerol)Blue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Human kappa chain.
Purity:
Purified by immunoaffinity chromatography followed by solid phase adsorption to remove any unwanted crossreactivities.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.. Reconstitute with 1ml sterile, distilled, deionized water or s
Specificity:
Recognizes human IgG Kappa. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Crossreactivity (by ELISA): Other Human heavy or light chain proteins: 1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.