Technical Data
I1904-40K
IgG, Fc (AP)
Description:
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:4500
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceRabbitGoat
Concentration:
~1mg/ml
Immunogen:
Rabbit IgG F(c) fragment
Purity:
Purified by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.05% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:
Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rabbit IgG, rabbit IgG F(c) and rabbit serum. No reaction was observed against rabbit IgG F(ab)2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.