		Technical Data
I1904-44A	IgG, Lambda (FITC)

Description:
Applications:
Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 495nm

Emission Wavelength: 528nm

Fluorochrome/Protein Ratio:
2.7 moles FITC per mole of Goat IgG

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		4°C (-20°C Glycerol)	Blue Ice	Human	Goat

Concentration:

~1mg/ml (UV280)

Immunogen:

Human lambda free chain.

Purity:

Purified by immunoaffinity chromatography using human antigens coupled to agarose beads followed by solid phase adsorption.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

Specificity:

Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.