Technical Data
I1904-44B
IgG, Lambda (Biotin)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:100,000
Western Blot: 1:2000 - 1:10,000
Immunohistochemistry: 1:1000 - 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human lambda light chain
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Specificity was confirmed by ELISA at less than 1% against other human heavy or light chain proteins
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.