Technical Data
I1904-44C
IgG, Lambda (HRP)
Description:
Applications:
Suitable for use in ELISA, Western and Dot blot, Immunoperoxidase electron microscopy, Immunohistochemistry, as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:16,000-1:64,000. Assayed against 1ug of human IgG
in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~1mg/ml (UV280)
Immunogen:
Human lambda light chain.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml 40-50% glycerol, ddH2O.
Specificity:
Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Crossreactivity: Other human heavy or light chain isotypes <1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.