		Technical Data
I1904-44D	IgG, Lambda (AP)

Description:
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Application(s): Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Applications: Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		4°C Do Not Freeze	Blue Ice	Human	Goat

Concentration:

~1mg/ml (UV280)

Immunogen:

Human lambda

Purity:

Prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Form

Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).

Specificity:

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-goat serum. Less than 1% crossreactivity against other human heavy or light chain isotypes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.