Technical Data
I1904-44R
IgG (Biotin)
Description:
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio: 1020 BAC molecules per Rabbit IgG molecule

Application(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Recommended Dilution(s): This product has been assayed against 1ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Storage Conditions: Store vial at 4 C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanRabbit
Concentration:
Antibody
Immunogen:
Human IgG (gamma chain)
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form
Biotin conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against other Human heavy or light chain proteins
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.