Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.
ELISA: 1:1600 to 1:16,000 using pNPP, p-nitrophenyl phosphate, as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.
Alkaline Phosphatase (Calf Intestine) (MW: 140kD)
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|1mg||4°C Do Not Freeze||Blue Ice||Human||Rabbit|
| ~1mg/ml (UV280)|
|Human IgG F(c) fragment|
|Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
|Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(ab’)2.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|