Technical Data
I1904-47J
IgG, H&L (AP)
Description:
Applications:
Suitable for use in ELISA, Dot Blot, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2500
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Optimal dilution to be determined by researcher.

Storage and Stability:

May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceRatGoat
Concentration:
~1mg/ml (UV280)
Immunogen:
Rat IgG whole molecule.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:
Recognizes rat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgG and Rat Serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.