Technical Data
IgG, Fc (AP)
Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:5000. assayed against 1ug of rat IgG in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at RT.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
1mg4C Do Not FreezeBlue IceRatGoat
~1mg/ml (UV280)
Rat IgG F(c) fragment
Prepared by immunoaffinity chromatography followed by solid phase adsorption.
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Recognizes rat IgG F(c) fragment. Does not crossreact with rat IgG F(ab)2. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rat IgG, rat IgG F(c) and rat serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.