		Technical Data
I1904-47Z	IgG, Fc (AP)

Description:
Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:5000. assayed against 1ug of rat IgG in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at RT.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		4°C Do Not Freeze	Blue Ice	Rat	Goat

Concentration:

~1mg/ml (UV280)

Immunogen:

Rat IgG F(c) fragment

Purity:

Prepared by immunoaffinity chromatography followed by solid phase adsorption.

Form

Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).

Specificity:

Recognizes rat IgG F(c) fragment. Does not crossreact with rat IgG F(ab’)2. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rat IgG, rat IgG F(c) and rat serum.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.