Technical Data
I1904-48K
IgG, IgA, IgM, H&L (FITC)
Description:
Applications:
Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label: Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 2.9 moles FITC per mole of Goat IgG

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4°C (-20°C Glycerol)Blue IceRatGoat
Concentration:
~2mg/ml (UV280)
Immunogen:
Rat IgG, IgA and IgM whole molecules
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes rat IgG, IgA, IgM, heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rat IgG, rat IgA and rat IgM. Suitable for detection of all rat isotypes and chain combinations.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.