Technical Data
I1904-49D
IgG, Fc (AP)
Description:
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution
ELISA: 1:500-1:3,000. 1ug of rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 min at RT.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceRatRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Rat IgG F(c) fragment
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab)2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Conjugation Reference: 1. Modified from Avarameas and Ternyrock, Immunohistochemistry 32: 1,175 (1971).