Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
ELISA: 1:500-1:3,000. 1ug of rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store at 4°C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
|1mg||4°C Do Not Freeze||Blue Ice||Rat||Rabbit|
| ~1mg/ml (UV280)|
|Rat IgG F(c) fragment|
|Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
|Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol.|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab’)2.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Conjugation Reference: 1. Modified from Avarameas and Ternyrock, Immunohistochemistry 32: 1,175 (1971).|