Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
|500ug||4°C Do Not Freeze||Blue Ice||Sheep||Rabbit|
|Sheep IgG whole molecule.|
|Purified by immunoaffinity chromatography. |
|Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Label: Alkaline phosphatase (Calf Intestine MW: 140kD).|
|Recognizes sheep IgG. Immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, sheep IgG and sheep serum.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Avarameas and Ternyrock, Immunochemistry 32: 1175 (1971).|