Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
ELISA: 1:5000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 500ug | | 4°C Do Not Freeze | Blue Ice | Sheep | Rabbit |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Sheep IgG whole molecule. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Label: Alkaline phosphatase (Calf Intestine MW: 140kD). |
| Specificity: |
| Recognizes sheep IgG. Immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, sheep IgG and sheep serum. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Avarameas and Ternyrock, Immunochemistry 32: 1175 (1971).
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