Technical Data
I1904-59D
IgG, H&L (AP)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store at 4 C before opening. DO NOT FREEZE. This product is stable at 4 C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug4C Do Not FreezeBlue IceSheepRabbit
Concentration:
~1mg/ml
Immunogen:
Sheep IgG whole molecule.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.1% sodium azide, 50% glycerol. Label: Alkaline phosphatase (Calf Intestine MW: 140kD).
Specificity:
Recognizes sheep IgG. Immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, sheep IgG and sheep serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Avarameas and Ternyrock, Immunochemistry 32: 1175 (1971).