Technical Data
I1904-65A1
IgG, Heavy Chain
Description:
The most common feature of plasmacytomas, and certain non-Hodgkin’s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:500-1:1000 for 30 min at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.

Positive Control: Tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
250ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
IgG isolated from a pool of normal human sera. Cellular Localization: Cytoplasmic
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes human IgG heavy chain at ~50kD. Does not crossreact with alpha (IgA), epsilon (IgE), mu (IgM), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.