Technical Data
I1904-65M
IgG (Biotin)
Description:
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Application(s): Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Recommended Dilution(s): This product has been assayed against 1.0 m g of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Storage Conditions: Store vial at 4 C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage mix product with 50% glycerol and then aliquot contents and freeze at-20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceMouseRabbit
Concentration:
1.0 mg/ml (UV280)
Immunogen:
Mouse IgG g heavy chain
Purity:
Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Form
Biotin conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against other Mouse heavy or light chain proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.