Technical Data
I1904-65T
IgG, Fc (AP)
Description:
Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:500-1:2500

Optimal dilutions to be determined by the researcher.

Label:
Alkaline Phosphatase (Calf Intestine) (MW: 140kD)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
Mouse IgG F(c) fragment
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide and 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against mouse IgG F(ab)2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.