Technical Data
I1904-66R
IgG, Kappa (HRP)
Description:
Applications:
Suitable for Immunoblot (Western and Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000-1:5000
ELISA: 1:50,000
Immunohistochemistry: 1:500-1-2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Mouse k (kappa) light chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (Immunoglobulin and Protease free), 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile distilled water or sterile 40-50% glycerol.
Specificity:
Recognizes mouse IgG k (kappa) light chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, mouse IgG and mouse serum. Crossreactivity against other mouse heavy and light chain isotypes (confirmed by ELISA): 1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.