Suitable for use in Immunoblot (Western or Dot Blot), ELISA and immunohistochemistry. Other applications not tested.
ELISA (Capture): 1:1000-1:2000. Assayed against 1ug of mouse IgG.
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Alkaline Phosphatase (Calf Intestine) (MW: 140,000D)
Storage and Stability:
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one year from date of opening product.
|1mg||4°C Do Not Freeze||Blue Ice||Mouse||Rabbit|
| ~1mg/ml (UV280)|
|Mouse k (kappa light chain)|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).|
|Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Solid phase adsorptions against a panel of mouse IgG lambda, IgM lambda and IgA lambda proteins was performed. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|